GCT-27
ACC 743
Cell line:
GCT-27
DSMZ no.:
ACC 743
Species:
human (Homo sapiens)
Cell type:
testicular germ cell tumor
Origin:
established from the germ cell tumor of a man with a malignant testicular teratoma (intermediate grade)
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
epithelioid cells growing adherently in monolayers; image
Medium:
80% RPMI 1640 + 20% h.i. FBS + 2 mM L-glutamin + 4 µg/ml human insulin + 400 ng/ml hydrocortisone
Subculture:
seed out at ca. 1-1.5 x 106 cells/175 cm2, split confluent culture 1:6 to 1:8 every 4-6 days using trypsin/EDTA; initially cells were cultivatd in collagen-coated flasks
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35-40 hours
Harvest:
cell harvest of ca. 7-8 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list