Leibniz Institute DSMZ: Details

Cell line:

WA-OSEL

DSMZ no.:

ACC 767

Species:

human (Homo sapiens)

Cell type:

chronic B cell leukemia

Origin:

established in 1993 from a peripheral blood sample of a man with B-type chronic lymphocytic leukemia; CD5 negative sister cell line of WA-C3CD5+ (DSM ACC 769)

Reference(s):

19334, 19172

Biosafety level:

1

Risk assessment:

The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 was negative by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects.

Permissions and restrictions:

A

Depositor-specific restrictions:

Transfer to third parties needs to be negotiated with the depositor.

DSMZ Cell Culture Data:

Morphology:

round cells growing singly in suspension and in large aggregates, also slightly adherent; image; image; image

Medium:

90% RPMI 1640 + 10% h.i. FBS

Subculture:

seed out at ca. 0.2 x 10⁶ cells/ml; optimal split ratio 1:3 - 1:5 twice a week; maintain at ca. 0.5-1 x 10⁶ cells/ml

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 40 hours

Harvest:

maximum density at ca. 1.8 x 10⁶ cells/ml

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in PCR assay

Immunology:

CD3 -, CD5 -, CD10 -, CD13 -, CD14 -, CD19 +, CD20 +, CD34 -, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa+, sm/cylambda -; image

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

human flat-moded hypotetraploid karyotype; 83-92<4n>XXYY, -6, -17, -18, del(11)(q22)x1-2, i(17)(q10)

Viruses:

PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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