UOC-M1
ACC 775
Cell line:
UOC-M1
DSMZ no.:
ACC 775
Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established at diagnosis in 1992 from the malignant bone marrow cells of a 68-year-old man with undifferentiated acute myeloid leukemia (FAB AML M1) of the megakaryoblastic lineage; cells are described in the literature to carry four copies of the KMT2A (MLL) gene
Reference(s):
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
round to elongated cells growing singly in suspension and some cells adherently; image
Medium:
90% McCoy´s 5a + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; optimal split ratio 1:2 every 2-3 days; maintain at ca. 0.5-1.5 x 106 cells/ml; adherent cells can be detached by tapping or using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-60 hours
Harvest:
maximum density at ca. 1.5-2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD4 -, CD7 +, CD13 +, CD14 -, CD15 -, CD19 -, CD24 +, CD33 +, CD34 +, CD38 +, CD41 +, CD42 -, cyCD68 +, HLA-DR +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypodiploid karyotype; -39-44<2n>XY, -7, -9, -11, -14, -19, +21, +21, der(Y)t(Y;9)(p11;p13)t(9;Y)(p24;p11), del(5)(q14q35), der(5)t(Y;5)(q11;p15)t(Y;9)(q12;q11), der(9;11)(11qter->q11::19p1?->p1?::9pter->q11::19p1?->p1?::11q11->q25::11q14->qter), der(17)t(7;17)(p15;p13), r(19)(p13q11), der(21)t(16;21)(p11;p11), der(21t(19;21)(p11;p11), der(21)trp(21)(q21.2q22.3)t(11;21)(q22;q22.3); copy number increase (to four per diploid genome) of unrearranged KMT2A (MLL) locus; resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list