Leibniz Institute DSMZ: Details

Cell line:

ETCC-006

DSMZ no.:

ACC 790

Species:

human (Homo sapiens)

Cell type:

breast ductal carcinoma

Origin:

established in 2010 from the tumor tissue biopsy of a 47-year-old woman with breast ductal carcinoma in situ (DCIS); cells were immortalized by stable transformation with human telomerase reverse transcriptase (hTERT); the ETCC-006 cell line is one of a series of five immortalized colonies from the same tumor sample (ETCC-006, ETCC-007 ((DSMZ ACC 791), ETCC-008, ETCC-010, ETCC-011); RNA sequence data are available (see Ref 40906 and RNA-Seq)

Reference(s):

18123, 40906

Biosafety level:

1, GMO-S1

Risk assessment:

The cell line ETCC-006 was established by stable lipofectamin transformation of breast carcinoma cells with the pGRN145 plasmid coding for the catalytic subunit of the human telomerase reverse transcriptase (hTERT) and for hygromycin resistence. Donor cells, vector und recipient cells match risik group 1

Permissions and restrictions:

A, D

Depositor-specific restrictions:

Transfer to third parties needs to be negotiated with the depositor.

DSMZ Cell Culture Data:

Morphology:

epithelioid cells growing adherently as monolayer; image; image

Medium:

90% RPMI 1640 + 10% h.i. FBS

Subculture:

seed out at ca. 1-2 x 10⁶ cells/80 cm² flask; split 90% confluent culture 1:2 to 1:6 once or twice a week using trypsin/EDTA (incubation with trypsin/EDTA for about 5 min)

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 2-4 days

Harvest:

cell harvest of ca. 3-4 x 10⁶ cells/80 cm²

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in PCR assay

Immunology:

To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database; identical to ETCC-007 (DSMZ ACC 791)

Molec. Genetics:

triple-negative due to absence of ER, PR and HER2 expression in Western blots

Viruses:

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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