PCL-12
ACC 794
Cell line:
PCL-12
DSMZ no.:
ACC 794
Species:
human (Homo sapiens)
Cell type:
chronic B cell leukemia
Origin:
established in 2012 from the peripheral blood of a 73 year-old man with chronic lymphocytic leukemia (CLL Rai stage I, Binet stage A) after multiple chemotherapeutic treatments; cells are described in the literature to highly express CD19 and CD5; however, DSMZ immunoprofiling showed that the level of CD5 expressing cells decrease to about 10% after long-term cultivation
Reference(s):
Biosafety level:
2
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were also positive by western blot in phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a at least inducible production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2.
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
round cells growing in big clusters in suspension
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; optimal split ratio 1:2 - 1:3 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-50 hours
Harvest:
maximum density at ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD 10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, HLA-DR +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list