MBU-IM
ACC 796
Cell line:
MBU-IM
DSMZ no.:
ACC 796
Species:
human (Homo sapiens)
Cell type:
keratinocytes
Origin:
established in 2011 from primary keratinocytes of a healthy neonatal foreskin; cells were immortalized by transduction with retroviral vectors containing human telomerase and SV40 early region
Reference(s):
Biosafety level:
1, GMO-S1
Risk assessment:
Primary keratinocyte cells isolated from a neonatal foreskin were transduced with replication deficient amphotropic retroviruses containing a) the catalytic subunit of human telomerase reverse transcriptase hTERT, and b) SV40 early region for immortalization; donor, vector, and recipient cells are categorized to biosafety level 1
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
100% Keratinocyte SFM + 0.2ng/ml hEGF + 15µg/ml pituitary extract
Subculture:
seed out at ca. 0.3 x 106 cells in a T75 flask; split 1:4 - 1:8 every 5-8 days using Accutase
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-60 hours
Harvest:
cell harvest of ca. 2-3 x 106 cells/75 cm2
Storage:
frozen with 90% Keratinocyte SFM, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV +
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list