MAPAC-HS-77
ACC 814
Cell line:
MAPAC-HS-77
DSMZ no.:
ACC 814
Species:
human (Homo sapiens)
Cell type:
pancreas ductal adenocarcinoma
Origin:
also named MaPac-77; established in 2015 from the resection of the head of the pancreas of a 64-year-old man suffering from pancreatic tumor (TNM stage pT3, pN1, L0 V0 Pn1; tumor grade G3; resection status R0)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
80% mixture of Ham's F12 + DMEM 4.5 g/l glucose (at 1:1) + 20% h.i. horse serum
Subculture:
seed out at ca. 2 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:5 once or twice a week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 2 days
Harvest:
cell harvest of ca. 30 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% horse serum, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in PCR assay
Immunology:
to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list