JVE-241
ACC 822
Cell line:
JVE-241
DSMZ no.:
ACC 822
Species:
human (Homo sapiens)
Cell type:
colon carcinoma
Origin:
established from the primary tissue of a mucinuous colon adenocarcinoma of the cecum (Dukes stage B2) of a 79-year-old man; cells were described in literature to carry mutations in the KRAS, APC and TP53 genes
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
round and polymorphic cells growing in suspension with the tendency to pile up and formation of clusters
Medium:
80% mixture of Ham's F12 + DMEM (1:1) + 20% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/25 cm2;
split growing culture 1:2 every 7-10 days; clusters may be difficult to count and to separate
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 8-10 days
Harvest:
cell harvest of ca. 6 x 106 cells/175 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list