HEK293-DR-GFP-RAD51B-9
ACC 845
Cell line:
HEK293-DR-GFP-RAD51B-9
DSMZ no.:
ACC 845
Species:
human (Homo sapiens)
Cell type:
embryonal kidney
Origin:
Genetic modification of HEK293-DR-GFP (DSMZ ACC 844, a hprtDR-GFP reporter cell line for studying chromosomal double-strand repair (DR)). This cell line carries a genetic disruption of RAD51B by frameshifting mutations in exon 3 (targeted by CRISPR-CAS9) and is puromycin resistant. The wild-type allele is no longer present. A sister cell line with the retrovirally reconstituted RAD51B gene is available as ACC 850
Biosafety level:
1, GMO-S1
Risk assessment:
genetically modified organism, according to German and European legislation allocated into group S1
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
Medium:
90% DMEM + 10% h.i. FBS + (additionally) 2 mM L-glutamine (to sustain genetic stability cells should be maintained, at least periodically, with 0.5 µg/ml puromycin)
Subculture:
culture vessels should be coated with 0.01% poly-L-lysine solution; seed out at 1 x 106 cells/25 cm2; split subconfluent culture 1:2 to 1:3 every 2-3 days using trypsin/EDTA; optimal density around 4 x 106 cells/25 cm2
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 48 hours
Harvest:
cell harvest of ca. 8 x 106 cells/25 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Molec. Genetics:
frameshift mutation in exon 3 of RAD51B was verified by sequence analysis
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list