TT2609-A02
ACC 859
Cell line:
TT2609-A02
DSMZ no.:
ACC 859
Species:
human (Homo sapiens)
Cell type:
follicular thyroid carcinoma
Origin:
established in 1996 from the tumor tissue of a 57-year-old man with local recurrence of follicular thyroid cancer (containing well-differentiated with dispersed poorly differentiated areas). Cells express the transcriptionally inactive (dominant negativ) delta-p53 isoform which is additionally mutated resulting in a truncated p53 form and a short 5 KDA fragment (delta p53 -/-). A sister cell line with an untruncated delta-p53 is available (ACC 510). Useful for studies with p53 family members p63 and p73
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
epithelioid cells growing adherently in monolayers
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS + 1 mM sodium pyruvate + 1x IST (5 µg/ml insulin + 5 µg/ml transferrin + 5 ng/ml sodium selenite)
Subculture:
seed out at about 1 x 106 cells/25cm2; split 1:2 to 1:3 once a week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 100 hours
Harvest:
cell harvest of ca. 15 x 106 cells/175 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: HBV -, HCV -, HIV-1 -, HIV-2 -, EBV -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list