UPCI-SCC-032
ACC 864
Cell line:
UPCI-SCC-032
DSMZ no.:
ACC 864
Species:
human (Homo sapiens)
Cell type:
squamous cell carcinoma
Origin:
retromolar trigone squamous cell carcinoma established in 1993 from the primary tumor tissue (grade 2, T2N2b) of a 60-year-old man. Cells are described to carry a chromosomal 11q13 amplification. The cell line will only be distributed to non-profit institutions, commercial entities may apply for a license from Maria Vanegas (msv4@pitt.edu / 001-414-648-4004) at the University of Pittsburgh, Office of Technology Management
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
The cell line will only be distributed to non-profit institutions. Requests for transfer to third parties and commercial entities may apply for licenses at the University of Pittsburgh, Office of Technology Management (Maria Vanegas; msv4@pitt.edu / 001-414-648-4004).
DSMZ Cell Culture Data:
Morphology:
Medium:
80% MEM (with Earle's salts) + 20% h.i. FBS + non-essential amino acids + 2 mM L-glutamine
Subculture:
seed out at about 0.5- 1 x 106 cells/80 cm2; split semi-confluent or confluent culture 1:2 to 1:4 every 3-5 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-60 hours
Harvest:
cell harvest of ca. 3-5 x 106 cells/80 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
to make inquiries about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list