GDM-1
ACC 87
Cell line:
GDM-1
DSMZ no.:
ACC 87
Species:
human (Homo sapiens)
Cell type:
acute myelomonocytic leukemia
Origin:
established in 1979 from the peripheral blood of a 66-year-old woman with a Philadelphia chromosome negative myeloproliferative disorder (resembling chronic myeloid leukemia, CML, in blast crisis) after transformation to refractory acute myelomonocytic leukemia (AML)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at 0.5-0.8 x 106 cells/ml; split 1:2 to 1:3 every 2-3 days (starting the culture in a 12- or 24-well plate may be of advantage)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
maximum density at 1.2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD4 +, CD13 -, CD14 -, CD15 (+), CD19 -, CD33 +, CD34 +, HLA-DR +; image
Authenticity:
STR analysis according to the global standard ASN-0002.1 resulted in an authentic STR profile of the
Cytogenetics:
human hyperdiploid karyotype - 48(46-48)<2n>XX, +8, +13, -16, +mar, t(2;11)(q36;q13), del(6)(q21), t(7;?)(q35;?), del(12)(p13)
Molec. Genetics:
expression of MNX1 (HLXB9) confirmed by RT-PCR and Western blot
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list