UPCI-SCC-182
ACC 887
Cell line:
UPCI-SCC-182
DSMZ no.:
ACC 887
Species:
human (Homo sapiens)
Cell type:
squamous cell carcinoma
Origin:
established in 1996 from a primary, moderately differentiated oral squamous cell carcinoma (stage 2, T2N1) of the oral retromolar trigone of a 71-year-old man.
Described as HPV negative and to carry a TP53 mutation (E294X), but no chromosomal 11q13 amplification
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
The cell line will only be distributed to non-profit institutions. Requests for transfer to third parties and commercial entities may apply for licenses at the University of Pittsburgh, Office of Technology Management (Maria Vanegas; msv4@pitt.edu / 001-414-648-4004).
DSMZ Cell Culture Data:
Morphology:
Medium:
90% MEM (with Earle's salts) + 10% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:4 every 4-8 days using trypsin/EDTA for 5-10 min (before trypsination wash 2x with PBS); initially after thawing cells may grow slowly
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 60-80 hrs (after recovery from liquid nitrogen ca. 100 hrs)
Harvest:
ca. 15 x 106 cells/175 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in PCR assay
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list