MaS-3
ACC 901
Cell line:
MaS-3
DSMZ no.:
ACC 901
Species:
human (Homo sapiens)
Cell type:
urinary bladder carcinosarcoma
Origin:
established in 2018 from tumor tissue of a
86-year-old male with carcinosarcoma of the urinary bladder after neoadjuvant radiotherapy
Reference(s):
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 with 4.5 glucose (5g/l) + 10% h.i. FBS
Subculture:
seed out at about 0.5-1 x 106 cells in a 80 cm2 flask; split confluent cukture 1:2 to 1:3 once or twice a week using trypsin/EDTA (wash 2x with PBS and then incubate for 5-10 min with trypsin)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 60-80 hours
Harvest:
cell harvest of ca. 3 x 106 cells/80 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list