P-19
ACC 316
Cell line:
P-19
DSMZ no.:
ACC 316
Species:
murine (mouse) (Mus musculus)
Cell type:
embryonal carcinoma
Origin:
established from the teratocarcinoma induced in a C3H/He strain mouse
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80-90% alpha-MEM (with ribo- and deoxyribonucleosides) + 10-20% h.i. FBS
Subculture:
split confluent culture 1:10 to 1:20 once or twice a week using trypsin/EDTA; seed out at ca. 0.3-0.4 x 105 cells/cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 2-3 days
Harvest:
cell harvest of max. 0.5 x 106 cells/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine near-diploid karyotype with 16% polyploidy - 40(31-44)<2n> - one centric fusion marker - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list