JIMT-1

ACC 589

Cell line:

JIMT-1

DSMZ no.:

ACC 589

Species:

human (Homo sapiens)

Cell type:

breast carcinoma

Origin:

established from the pleural effusion of a 62-year-old woman with ductal invasive breast cancer (grade 3 invasive, T2N1M0) after postoperative radiation in 2003; cell line was described in the literature to carry an amplified HER-2 oncogene and to be insensitive to HER-2-inhibiting drugs, e.g. trastuzumab (Herceptin); listed in the CCLE (ref 18232); RNA sequence data are available (see Ref 40906 and RNA-Seq)

Reference(s):

15007, 18256, 18257, 18232, 40906

Biosafety level:

Permissions and restrictions:

DSMZ Cell Culture Data:

Morphology:

epithelial-like cells growing in monolayers; image; image

Medium:

90% DMEM + 10% h.i. FBS

Subculture:

seed out at ca. 1 x 10⁶ cells/80 cm²; split confluent culture 1:2 to 1:8 every 2-4 days using trypsin/EDTA; during the first 1-2 weeks cell growth may be rather slow

Incubation:

at 37 °C with 10% CO₂

Doubling time:

ca. 30-40 hours

Harvest:

cell harvest of ca. 8-10 x 10⁶ cells/80 cm²

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in microbiological culture, PCR assays

Immunology:

To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

human flat-moded highly rearranged hyperdiploid karyotype with 6% polyploidy - 46-56<2n>XX, +1, +2, +2, +3, +5, +6, +7, +12, -13, -14, -15, -15, -16, -18, +17, +5mar, add(X)(p22.1), add(1)(q11), del(1)(q32.2), der(1)add(1)(p21)add(1)(q22), der(1)t(1;6)(p11;q1?1)t(6;5)(q25;?p13), add(2)(q11)x2, t(2;3)(q10;p10)add(2)(q25-31), add(3)(p11), der(4)t(?3;4)(?q24;p11), add(5)(q15), der(5)add(5)(p15.3)del(5)(q22), der(5)t(5;8)(p11;p11), add(6)(p11)add(6)(q23), der(7)t(1;7)(p13;q21), der(7)t(7;?12)(p11;p11), add(8)(p11), i(8q) add(9)(p11), der(9)add(9)(p11)add(9)(q23), add(11)(p13), der(11)dup(11)(p11p15)add(11)(p15), der(11)t(1;11)(q24;p12), add(12)(q21), der(12;?15)(p11;q11), add(17)(p11), add(17)(p13), der(17)add(17)(p11)add(17)(q21), inc; unresolved HSR markers present

Molec. Genetics:

Western blot : ER, PR, AR negative while positive for HER2

Viruses:

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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