MH1C1
ACC 106
Cell line:
MH1C1
DSMZ no.:
ACC 106
Species:
rat (Rattus norvegicus)
Cell type:
hepatoma
Origin:
a clonal strain of epithelial cells was derived from a transplantable Morris hepatoma (#7795, Buffalo strain) in 1967; cells were described in the literature to synthesize and to secrete serum albumin and serum complement component C9
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
85-90% DMEM + 10-15% h.i. FBS
Subculture:
split confluent culture 1:2 to 1:4 about once or twice per week using trypsin/EDTA; seed out at ca. 2 x 106 cells/75 cm2
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 33-60 hours
Harvest:
cell harvest of about 20 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species.
Cytogenetics:
near-diploid rat karyotype with 10% polyploidy - 44(40-46)<2n>
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list