L138.8A
ACC 114
Cell line:
L138.8A
DSMZ no.:
ACC 114
Species:
murine (mouse) (Mus musculus)
Cell type:
mast cell
Origin:
established from bone marrow mast cells of a female BALB/c mouse in 1984
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
cells are initially (after thawing) difficult to grow! after thawing seed at different concentrations in 24-well plates, later maintain at 0.1-1.0 x 106 cells/ml, do not exceed 1 x 106 cells/ml; split 1:4 to 1:10 about 1-2 times per week.
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
maximal density at about 2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list