Species:
human (Homo sapiens)
Cell type:
chronic myeloid leukemia in blast crisis
Origin:
established from the peripheral blood of a 3-year-old girl with chronic myeloid leukemia (CML) in blast crisis in 1975; cells were described in the literature to be TdT-positive and to carry the Philadelphia chromosome t(9;22) leading to BCR-ABL1 fusion gene. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
cells are difficult to culture! Seed out at ca. 2 x 106 cells/ml; cells grow initially slowly; it might be of benefit to start culture in 24-well plates; maintain at 1.0-2.0 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48 hours
Harvest:
maximal density at about 3-5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3-, CD10+, CD13-, CD19+, CD20(+), CD34-, CD37+, CD38+, CD80-, CD138-, HLA-DR+, sIgG-, sIgM+, cIgG-, cIgM+, sIgkappa-, sIglambda-, cIgkappa-, cIglambda-;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypodiploid karyotype with 5% polyploidy - 45(42-47)<2n>X, -X, der(9)t(9;22)(q34;q12)x1-2, dup(13)(q21), der(22)t(9;22)(q34;q12) - identity confirmed
Molec. Genetics:
expression of fusion gene BCR-ABL1 e13-a2 (b2-a2) confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
