SIMA
ACC 164
Cell line:
SIMA
DSMZ no.:
ACC 164
Species:
human (Homo sapiens)
Cell type:
neuroblastoma
Origin:
established from the adrenal tumor tissue resected after treatment from a 20-month-old boy with neuroblastoma (stage III) in 1991
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS + 2 mM L-glutamine
Subculture:
seed out at ca. 3-5 x 106 cells/80cm2 or 0.4 x 106 cells/ml; split confluent culture 1:3 to 1:5 every 3-6 days using trypsin/EDTA or by tapping the flask; cells may grow slowly initially forming non-adherent large cell aggregates
Incubation:
at 37 °C with 5% CO2
Doubling time:
highly variable about 34-100 hours
Harvest:
cell harvest of about 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypotetraploid karyotype with 3.5% polypoidy - 88/89<4n>XXY/XXYY, -Y, -11, -17, -17, der(1qh++)t(1;17)(p34-35;q12)x2, t(6;9)(p21;q33)x2, ?inv(12)(q21q24)x2, der(22)t(17;22)(q21;p12)x2 - ca. 50-100 NMYC-containing dmin present in each cell - t(1;17) together with NMYC amplification is restricted to neuroblastoma - matches published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list