COLO-680N
ACC 182
Cell line:
COLO-680N
DSMZ no.:
ACC 182
Species:
human (Homo sapiens)
Cell type:
esophageal squamous cell carcinoma
Origin:
derived from the esophagus squamous cell carcinoma of a 58-year-old woman in 1985 via passage through a nude mouse
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2; split confluent cultures 1:2 to 1:3 every 2-3 days using trypsin/EDTA;
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 60-70 hours
Harvest:
cell harvest of about 10-12 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human highly rearranged hyperdiploid/hypotriploid karyotype with 13.5% polyploidy - 53-58<2n>X, -X, +1, +1, -3, -5, -5, +6, +7, +12, -13, -14, -14, -16, -16, -18, -20, -21, -22, +16mar - add(1)(p35)x1-2, add(1)(q11)x1-2, add(1)(p11), add(3)(p11), add(4)(q35), add(7)(q35)x1-2, add(7)(p22)del(7)(q11q21), der(9)t(5;9)(q14;p11), del(11)(p11), add(12)(q24), add(19)(p13), add(19)(q13)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list