CAL-39
ACC 187
Cell line:
CAL-39
DSMZ no.:
ACC 187
Species:
human (Homo sapiens)
Cell type:
vulva squamous cell carcinoma
Origin:
established from the primary lesion resected from a 76-year-old woman with bulky, moderately differentiated squamous cell carcinoma of the vulva in 1984; cells were reported to express an elevated number of high affinity EGF receptors, to carry gene amplification at 11q13 and to be tumorigenic in nude mice
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% DMEM + 20% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 0.5 nM hydrocortisone + 1 µg/100 ml EGF
Subculture:
cells are difficult to culture! Seed out 0.8-1 x 106 /80 cm2; split confluent culture 1:3 to 1:4 every 5-10 days using trypsin/EDTA (cells may need 10 min or even longer exposure to trypsin)
Incubation:
at 37 °C with 10% CO2
Doubling time:
about 50-70 hours
Harvest:
cell harvest of about 4-6 x 106cells /175cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypotriploid karyotype with 27% polyploidy - 59(54-60)<3n>XX, -X, +1, +1, +2, +3, +9, +12, +12, +16, +20, +22, +4mar, del(1)(q22), del(3)(p14), add(4)(p16), del(7)(q32), add(9)(p22), del(12)(q15), add(12)(q24), add(18)(q23) - additional 11q rearrangements present in markers - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list