COLO-699 - Discontinued
ACC 196
Cell line:
COLO-699 - Discontinued
DSMZ no.:
ACC 196
Species:
human (Homo sapiens)
Cell type:
melanoma (cell line RPMI-7932)
Origin:
Caution: STR analysis demonstrated that cell line COLO-699 is genetically identical to human melanoma cell line RPMI-7932. Cell line COLO-699 must not be used as model for lung adenocarcinoma.
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
epitheloid cells growing adherently as monolayer
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out about 1-2 x 106 cells/175 cm2; split confluent cultures every 2-3 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35-40 hours
Harvest:
cell harvest of about 12 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in the STR profile of cell line RPMI-7932.
Cytogenetics:
human hypertriploid karyotype with 2% polyploidy - 70(62-72)<3n>XX, -X, +1, +3, -4, +6, +7, +7, +8, -9, -10, +12, -14, -16, -17, -17, +18, +20, der(2)t(2;10)(q27;q12), der(7)t(7;?)(p21;?)x2, i(10q), der(11)t(8;11)(q12;q14), idic(16q), der(21)t(21;?)(p12;?)x2 - a supernumerary small ring chromosome was present in 28% cells - corresponds to original karyotype with reinterpretation
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as: