IGR-37
ACC 237
Cell line:
IGR-37
DSMZ no.:
ACC 237
Species:
human (Homo sapiens)
Cell type:
melanoma
Origin:
established from the lymph node metastasis (groin) of a 26-year-old man with malignant melanoma (primary tumor histology: SSM level IV); cell line IGR-39 from the primary tumor is available (DSMZ ACC 239); described as strong tumorigenic in nude mice
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
85% DMEM + 15% h.i. FBS
Subculture:
seed out at ca. 2.5 x 106 cells/75 cm2; split confluent culture 1:2 every 7-14 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca.48-72 hours
Harvest:
cell harvest of about 2-3 x 105/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, matching the profile of sister cell line IGR-39
Cytogenetics:
human flat-moded hypertriploid karyotype with 11% polyploidy - 78(67-78)<3n>XXY, +1, +3, +5, +7, +7, +8, +9, -10, -12, -13, -14, -15, -17, +18, +20, +20, +2mar, der(1)t(1;?21)(p13;q11)x2, del(9)(p13p21), der(10)t(9;10)(q11/21;q23/25), i(13q)x1-2, der(16)t(1;16)(p11/13;q21/23)x2, der(19)t(17;19)(q12;p13)x1-2 - rearrangements at 1p12/22, 19p13 and +7 recur in malignant melanoma
Molec. Genetics:
Genomic sequencing and/or ARMS assay show that the cell line carries the BRAF V600E mutation (mu/wt), but no NRAS Q61 mutation
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list