BEN
ACC 254
Cell line:
BEN
DSMZ no.:
ACC 254
Species:
human (Homo sapiens)
Cell type:
lung carcinoma
Origin:
established from the supraclavicular tumor cells-containing lymph node of a 71-year-old man with poorly differentiated carcinoma of the lung, classified as large cell carcinoma; cells cary a complex karyotype; listed in the CCLE (ref 18232)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% DMEM (4.5 g/L glucose) + 10% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:3 every 3-4 days using trypsin/EDTA
Incubation:
at 37 °C with 10% CO2
Doubling time:
about 40-50 hours
Harvest:
cell harvest of about 20 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human highly rearranged flat-moded near-tetraploid karyotype - 82-92<4n>X/XY, -X, -Y, -Y, -2, -2, -3, -3, -4, -4, -5, +6, +6, +7, -8, -8, -9, -10, -13, -13, -14, -16, -16, -17, -18, -18, -19, -21, -21, -21, -22, +18mar - del(1)(q22)x2, add(1)(p11), i(2q), i(4q), add(5)(p11), del(6)(q13)x3, del(6)(p21.2), der(7)(t(7;12)(p11;q11), del(7)(q21)x2, i(7p), del(8)(p21), del(9)(p12), del(10)(q24), del(11)(p11)x2, add(12)(q24), i(12q), del(?12)(q14), add(14)(p11), add(18)(q21/23), add(19)(p13)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list