HUP-T3
ACC 259
Cell line:
HUP-T3
DSMZ no.:
ACC 259
Species:
human (Homo sapiens)
Cell type:
pancreas carcinoma
Origin:
established in 1984 from the malignant ascites of a 66-year-old man with a poorly differentiated adenocarcinoma of the body and tail of the pancreas; described to be tumorgenic in nude mice
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% MEM (with Earle's salts) + 10% h.i. FBS + 1 mM non-essential amino acids + 1 mM sodium pyruvate
Subculture:
seed out about 1 x 106 cells/80 cm2; split confluent culture 1:2 to 1:4 every 3-4 days
using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 70 hours
Harvest:
about 14 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypodiploid karyotype with 13% polyploidy - 39(36-40)<2n>XY, -6, -8, -9, -10, -12, -13, -17, -17, -19, -20, +3mar (2 rings), del(4)(p15), add(11)(q22.3;HSR), add(12)(q24;HSR), add(19)(p13), add(21)(p12) - gene amplification suggested by large HSR - ch 17 nullisomy
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list