Species:
human (Homo sapiens)
Cell type:
T cell acute lymphoblastic leukemia
Origin:
established from the peripheral blood of a 16-year-old woman with acute lymphoblastic leukemia (ALL) in 1972. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Morphology:
round cells growing singly or in small clumps in suspension;
image;
image;
image
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at about 0.5 - 0.7 x 106 cells/ml; maintain between 0.3 - 1.5 x 106 cells/ml;
split ratio of 1:3 to 1:6 once or twice a week
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 48 hours
Harvest:
saturation density at about 1-1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
initial contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays
Immunology:
CD2-, CD3-, CD4-, CD5+, CD6(+), CD7(+), CD8-, CD13-, CD19-, CD34+, TCRalpha/beta-, TCRgamma/delta-;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypotetraploid karyotype with 2% polyploidy - 90(79-91)<4n>XXX, -X, +3, +3, -10, -13, -14, +15, -18, -20, +2mar, dup(4)(q13q23)x2, del(6)(q14q22)x2, t(11;14)(p15;q11)x2, add(15)(p13) - sideline with der(1)t(1;9)(p35/36;q11), add(13)(q34) - carries t(11;14) with LMO1-TRD (LMO1-TCRD) rearrangement and cryptic del(1)(p32) effecting STIL-TAL1 (SIL-TAL1) fusion, resembles published karyotypes
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
