JTC-27
ACC 330
Cell line:
JTC-27
DSMZ no.:
ACC 330
Species:
rat (Rattus norvegicus)
Cell type:
hepatoma
Origin:
established from the transplantable rat ascites hepatoma AH-601 induced by feeding with aminoazotoluene; the hepatoma was passaged by intraperitoneal transplantation though JAR-2 rats
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
split confluent culture 1:6 to 1:8 twice a week using trypsin/EDTA; seed out at ca. 2 x 104 cells/cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 37 hours
Harvest:
cell harvest of maximally 2.5-3 x 105 cells/cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI,microbiological culture, RNA hybridization assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species.
Cytogenetics:
rat hyperdiploid karyotype with 15% polyploidy - 47(45-49)<2n>
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list