P12-ICHIKAWA
ACC 34
Cell line:
P12-ICHIKAWA
DSMZ no.:
ACC 34
Species:
human (Homo sapiens)
Cell type:
T cell leukemia
Origin:
established from the peripheral blood of a 7-year-old boy with acute lymphoblastic leukemia (ALL)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at 0.3-1 x 106 cells/ml; optimal split ratio 1:3 to 1:5
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
maximum density at about 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA, PCR hybridization assays
Immunology:
CD2 +, cyCD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypotetraploid karyotype with 1.6% polyploidy - 82-84<4n>XX, -Y, -Y, -2, +6, -9, -9, -10, -14, +20, -21, -21, -22, -22, +mar, der(2;9)(p10;q10), del(4)(q25), der(10;22)(q10;q10), add(19)(q13) - sideline with N4, add(8)(p1?) - matches published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list