Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established from the peripheral blood taken in 1991 at relapse of a high-grade B-NHL (leukemic transformation of mantle cell lymphoma, stage IV) diagnosed in a 58-year-old woman with previous history of cervical carcinoma. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was detected by western blot after stimulating the cells with phorbol ester / sodium butyrate. The infection was classified as potentially lytic associated with a possible production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2.
Permissions and restrictions:
Medium:
90% DMEM (4.5 g/L glucose) + 10% h.i. FBS + 2 mM L-glutamine
Subculture:
seed out at ca. 1.0 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; split ratio of 1:3 to 1:4 every 3 days
Incubation:
at 37 °C with 10% CO2
Doubling time:
about 48 hours
Harvest:
maximum density of about 2.6 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays
Immunology:
CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypodiploid karyotype with 8% polyploidy - 44(39-44)<2n>XX, -12, -17, -18, +mar, add(1)(p22), del(3)(p14p23), i(8p), i(8q), add(9)(p22)x1-2, t(11;14)(q13;q32), add(13)(p12), add(18)(q21) - sideline with two copies of der(14) and der(9) - carries t(11;14) and rearrangement at 9p22 associated with CCND1 (cyclin D1) activation and deletion of p15/p16 - matches published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
