Species:
human (Homo sapiens)
Cell type:
anaplastic large cell lymphoma
Origin:
established from the pleural effusion of a 10-year-old boy in 1973; original diagnosis was diffuse histiocytic lymphoma, corrected to diffuse large cell lymphoma (1984) and malignant histiocytosis (1989); according to REAL/WHO classification considered to represent an anaplastic large cell lymphoma (ALCL); described in the literature to carry the t(2;5)(p23;q35) NPM1-ALK (NPM-ALK) fusion gene. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.3 x 106 cells/ml; maintain at 0.3-1.0 x 106 cells/ml; split 1:2 to 1:3 every 2-3 days; culture may contain a certain amount of debris (which can be removed by centrifugation)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-50 hours
Harvest:
maximal density at ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD5 +, CD10 -, CD13 -, CD19 -, CD20 -, CD25 +, CD30 +, CD34 -, CD37 -, CD38 -, CD71 +, CD80 -, CD138 -, HLA-DR -, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypertriploid karyotype with 16% polyploidy - 74(67-75)<3n>XX, -Y, +1, +2, +3, +5, -7, +12, -16, -18, +19, -20, +21, +3mar, del(1)(p21), t(2;5)(p23;q35)x2, del(6)(q23)x1-2, add(8)(p12), add(9)(p21), del(10)(p14), add(12)(q24), add(14)(p12), add(16)(q24), dup(19)(q13.1qter) - sideline with der(12)t(12;19)(q13;q13) - carries two copies of balanced t(2;5) associated with ALCL - matches published karyotype - expression of NPM1-ALK (NPM-ALK) fusion gene was detected by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
