FDCP-1
ACC 368
Cell line:
FDCP-1
DSMZ no.:
ACC 368
Species:
murine (mouse) (Mus musculus)
Cell type:
bone marrow
Origin:
established from the long-term bone marrow culture of B6D2F1 mice; cells are IL-3-dependent; their differentiation is blocked; this subclone is responsive to G-CSF (FDCP means "Factor-Dependent Continuous cell lines, Paterson Laboratories" after the Paterson Insitute, Manchester, UK)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
single or clustered cells growing in suspension; image
Medium:
70% Iscove's MDM + 20% horse serum + 10% vol conditioned medium of WEHI-3B cell line (DSM ACC 26), or alternatively 10 ng/ml mIL-3
Subculture:
split saturated culture 1:2 to 1:3 every 2-3 days; seed out at ca. 5 x 105 cells/ml, first 3-4 days after thawing should not be splitted. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines.
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
maximal density at ca. 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine hypodiploid karyotype - 32<2n> - carries 8 centric fusion markers
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list