Species:
human (Homo sapiens)
Cell type:
chronic myeloid leukemia in myeloid blast crisis
Origin:
established from the peripheral blood of a 38-year-old man with chronic myeloid leukemia (CML) in myeloid blast crisis; described in the literature to express basophilic features; carries t(9;22) leading to BCR-ABL1 e14-a2 (b3-a2) fusion gene. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
cells grow very slowly; seed out at ca. 0.5 x 106 cells/ml; after thawing, cells may have a lag phase of 1-2 weeks before reassuming vigorous growth; then, maintain at 0.4-0.8 x 106 cells/ml; split 1:2 every 3-4 days; viability may not exceed 80%
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 80-100 hours
Harvest:
maximum density of ca. 1.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD13 +, CD14 -, CD15 -, CD33 +, CD71 +, CD235a +;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypotriploid karyotype with 8% polyploidy - 61(58-62)<3n>XYY, -2, -3, -5, +6, -7, +8, -10, -12, -16, -17, -18, +19, -20, t(9;22)(q34;q11)x2, i(11q), i(17q) - Ph (two copies) together with i(17q) and additional chs 8 and 19 - matches published karyotype
Molec. Genetics:
expression of fusion gene BCR-ABL e14-a2 (b3-a2) confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
