CMK

human (Homo sapiens)

acute megakaryocytic leukemia

established from the peripheral blood of a 10-month-old boy with Down's syndrome and acute megakaryocytic leukemia (AML M7) at relapse in 1985. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)

14685, 14921, 18187

1

A

Distribution to academia only.

single, multiformed, large cells growing in suspension, sometimes loosely adherent (<3%); 2-3% giant, multinucleated cells; image; image; image; image

80% RPMI 1640 + 20% h.i. FBS

seed out at ca. 0.5 x 10⁶ cells/ml; maintain at 0.3-1.0 x 10⁶ cells/ml; split saturated culture 1:2 to 1:4 every 2-4 days; viability may be consistently between 60% and 80% and culture may always contain a certain amount of cell debris; only exchange spent medium partially during the first week; cultivation in 12- or 24-well plates could be of advantage

at 37 °C with 5% CO₂

ca. 40-50 hours

maximal density at ca. 1.0-1.5 x 10⁶ cells/ml

frozen with 70% medium, 20% FBS, 10% DMSO

negative in DAPI, microbiological culture, RNA hybridization, PCR assays

CD3 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, CD71 +, CD235a +; image

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

human flat-moded hypotetraploid karyotype with 8% polyploidy - 85-90<4n>XY, -X, -Y, -2, -3, +5, -6, -6, -8, +11, -15, -15, +16, -17, -19, +21, +22, +7-11mar, add(1)(q31), add(1)(p36), add(3)(q11), del(3)(p14)x2-3, add(5)(q11), add(5)(q13), dup(8)(q11q21), add(8)(q13-21), del(8)(q11), del(9)(p21)x2, add(9)(q11)x2, del(10)(q22q24), der(11;17)(q10;q10), der(11)dup(11)(p13p15)t(5;11)(q11;p15)x1-2, del(11)(q23), add(12)(p13)x2, add(17)(p1?), add(18)(q23)x2-3, add(19)(p13), der(20)t(1;20)(q2?5;q1?2)x2, add(22)(q13) - disomic rearrangement of 20q1 close to common deleted segment, together with additional rearrangements of ch 20 present in markers - matches published karyotype

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Delivery form		Prices
Frozen culture		450,- €
Growing culture   (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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