LOU-NH91
ACC 393
Cell line:
LOU-NH91
DSMZ no.:
ACC 393
Species:
human (Homo sapiens)
Cell type:
lung squamous cell carcinoma
Origin:
established from the lower right lung lobe removed two months after diagnosis of highly differentiated squamous cell lung carcinoma with metastasis to one lymph node (no visceral involvement) in a 64-year-old woman (with any prior therapy); listed in the CCLE (ref 18232)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
adherent ovoid-to-epitheloid cells growing in monolayers; image
Medium:
80% RPMI 1640 + 20% h.i. FBS + 2 mM L-glutamine
Subculture:
seed out at ca. 0.5-1.0 x 106 cells/80 cm2; initially, growing may be slow; later, split confluent culture 1:2 to 1:4 every 5-10 days using trypsin/EDTA (incubation time may be 5 min or longer)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 80 hours
Harvest:
cell harvest of ca. 1.0-1.5 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with declining hyperhaploid sideline (2%) and 9% polyploidy - 50-53<2n>XX/XXXX, +5, +5, +7, +7, -13, +17, -18, +22, +22, +1-2mar, del(4)(q27), ?add(22)(q13)x2 (major clone) - 54<2n>XXXX, +5, +5, +7, +7, +22, +22 (minor clone) - 27XX, +5, +7, +22 - matches published karyotype - unstable karyotype with recent gain of structural rearrangements
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list