BHY

ACC 404

Cell line:

BHY

DSMZ no.:

ACC 404

Species:

human (Homo sapiens)

Cell type:

oral squamous cell carcinoma

Origin:

established from the tumor of a 52-year-old man with highly differentiated squamous cell carcinoma of the lower alveolus which was highly invasive to the mandibular bone and the muscle layer of the oral floor; described as anchorage-independent and to be tumorigenic in nude mice

Reference(s):

14652

Biosafety level:

Permissions and restrictions:

DSMZ Cell Culture Data:

Morphology:

epithelial-like polygonal or round and flat cells (sometimes spindle-form, very heterogenous) growing in monolayers; image; image; image; image

Medium:

90% DMEM (4.5 g/L gluocse) + 10% h.i. FBS

Subculture:

seed out at ca. 1 x 10⁶ cells in a T75 flask in 15 ml medium; split confluent culture 1:2 to 1:3 every 3-5 days using trypsin/EDTA

Incubation:

at 37 °C with 10% CO₂

Doubling time:

ca. 70 hours

Harvest:

cell harvest of ca. 7 x 10⁶ cells/175 cm²

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

initial contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays

Immunology:

To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

human flat-moded hypotriploid karyotype with 28% polyploidy - 61-68<3n>XX, -Y, -1, -2, +4, -5, -6, +9, +11, -13, +15, -17, -18, +20, +20, -21, +3-4mar, del(X)(q11q21), add(1)(q11), ?inv(2)(p17q11), add(2)(q11), del(3)(p11p14.3)x2, i(4p), add(4)(q3?1), del(5)(q13), add(5)(p15)x1-2, der(8;10)(q10;q10), add(9)(q34), add(9)(p11), del(10)(p13), del(10)(q23), der(11)t(1;11)(q11;q25), add(12)(p11), add(12)(p11), add(12)(q13-21), add(13)(p11), add(16)(q24), del(16)(p13), add(17)(p12), add(18)(p11), add(19)(q13) - 3-10 dmin counted in some cells

Viruses:

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV+, SMRV -

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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