RLD-1
ACC 415
Cell line:
RLD-1
DSMZ no.:
ACC 415
Species:
murine (mouse) (Mus musculus)
Cell type:
T cell leukemia
Origin:
established from transplantable tumors induced in syngeneic mice inoculated with cell suspensions prepared from lymph nodes infiltrated by lymphatic leukemia induced in 6-week-old DBA/2 mice by Rauscher virus (present in supernate of JLSV-9 cells); described as expressing T cell surface markers (Thy-1, T-200, Lyt-1), high constitutive TdT activity, and as differentiating along T-helper lineage in response to treatment with DMSO or phorbol ester TPA
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.3-0.5 x 106 cells/ml; maintain at 0.2-0.7 x 106 cells/ml; optimal split ratio of 1:2 to 1:6 every 2-4 days; cell growth may be slow for the first few days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 20-30 hours
Harvest:
saturation density of ca. 1.5-2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine hyperdiploid karyotype - 42<2n>XX, +14, +15 - no centric fusion markers present - matches published karyotype
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list