RED-4
ACC 425
Cell line:
RED-4
DSMZ no.:
ACC 425
Species:
murine (mouse) (Mus musculus)
Cell type:
erythroid leukemia
Origin:
established from a Rauscher murine leukemia virus-induced erythroid leukemia of a DBA/2 mouse; in contrast to RED-1 (DSM ACC 407), cells were described in the literature to be unresponsive to erythropoietin (EPO) or DMSO
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
85-90% DMEM + 10-15% h.i. FBS
Subculture:
maintain at 0.5 x 106 cells/ml; split saturated culture at 1:2 to 1:3 every day; seed out at ca. 1.0 x 106 cells/ml; viability immediately after thawing may be rather low (<30%), but cells recover subsequently over the next week and viability will raise over the next few passages (spent medium should be exchanged with discarding cells); cells grow better in 24-well-plates; maximal density at ca. 1.0 x 106 cells/ml
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 24 hours
Harvest:
cell harvest of ca. 1.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine near diploid karyotype with 12.5% polyploidy - 39-42<2n>XX, Df(2E2F1) - 1-2 centric fusion markers present - resembles published karyotype
Viruses:
ELISA: reverse transcriptase positive
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list