MOLT-14
ACC 437
Cell line:
MOLT-14
DSMZ no.:
ACC 437
Species:
human (Homo sapiens)
Cell type:
T cell leukemia
Origin:
established from the bone marrow of a 2-year-old girl with T-ALL (T cell acute lymphoblastic leukemia, FAB L2) at relapse in 1983; MOLT-13 (ACC 436) is a simultaneous sister cell line derived from the same patient sample with similar or identical features; by STR analysis, MOLT-13 and MOLT-14 are genetically identical
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5-1 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; split saturated culture 1:4 to 1:5 twice a week; culture medium becomes rarely acidic (yellow)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-50 hours
Harvest:
maximal density at ca. 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 +, CD5 +, CD7 +, CD8 -, CD13 -, CD19 -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, identical to that of MOLT-13
Cytogenetics:
human pseudodiploid karyotype with 8% polyploidy - 46(44-48)<2n>XX, der(17)t(17;17)(p13;q22.1) - resembles published karyotype - karyotypically identical to MOLT-13
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list