FU-OV-1
ACC 444
Cell line:
FU-OV-1
DSMZ no.:
ACC 444
Species:
human (Homo sapiens)
Cell type:
ovarian carcinoma
Origin:
established post-hysterectomy from the tumor tissue of a 65-year-old woman with malignant ovarian carcinoma (stage: FIGO IIIc; histology: poorly differentiated serous papillary adenocarcinoma) in 1989
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80-90% mixture of Ham's F12 + DMEM (at 1:1) + 10-20% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/175 cm2; split confluent culture 1:2 to 1:3 once a week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 120 hours
Harvest:
cell harvest of ca. 5-10 x 106 cells/175 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypotriploid karyotype with 12% polyploidy - 58-62<3n>X, -X, -X, -1, -2, -3, -4, -7, +9, -10, -12, -13, -14, -16, -17, -17, -18, -19, -20, -20, -22, +fra, +dm, del(2)(p23), inv(3)(p25q29), del(4)(q21/22), add(5)(p11), add(6)(q27), add(9)(p11), add(11)(p15)x2, add(11)(q24), add(14)(p11), add(14)(p11), add(16)(p13), add(19)(q13)x2, add(?21)(p11)x2 - resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list