WMP-2
ACC 453
Cell line:
WMP-2
DSMZ no.:
ACC 453
Species:
murine (mouse) (Mus musculus)
Cell type:
lymphoblasts
Origin:
established from spleen and lymph nodes of an adult Maloney murine leukemia virus (MoMuLV)-infected female WMP/WMP wild mouse; cells are described in the literature to carry nine pairs of metacentric Robertsonian translocation chromosomes containing 1 to 18; cells can be used for physical gene mapping by in situ hybridization in mice
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% RPMI 1640 + 20% h.i. FBS + 50 µM mercaptoethanol
Subculture:
split saturated culture at 1:3 to 1:5 every 2-4 days; seed out at ca. 2.5 x 105 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-40 hours
Harvest:
cell harvest of ca. 2.0-2.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine karyotype with a preponderance of centric fusion markers - resembles published karyotype
Viruses:
PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list