CL-11
ACC 467
Cell line:
CL-11
DSMZ no.:
ACC 467
Species:
human (Homo sapiens)
Cell type:
colon carcinoma
Origin:
established from the primary tumor at the left colon (a moderately differentiated colorectal carcinoma of TNM stage 3); sister cell line of mucoid growing cell line CL-11M; cells were described as expressing tumor-associated carcino-embryonic antigen (CEA)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% mixture of Ham's F12 + DMEM (at 1:1) + 20% h.i. FBS
Subculture:
seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:2 to 1:5 once or twice a week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 3-4 days
Harvest:
cell harvest of ca. 12 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-diploid karyotype with 12% polyploidy - 46(45-47)<2n>XY, -6, +7, -17, -18, +2-3 mar, +1-4 supernumerary r/dm, add(8)(q24) - sideline with idem, +7
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list