RH-30
ACC 489
Cell line:
RH-30
DSMZ no.:
ACC 489
Species:
human (Homo sapiens)
Cell type:
rhabdomyosarcoma
Origin:
established from the bone marrow metastasis of a 17-year-old man with undifferentiated (standard classification) or unclassified (Palmer classification) alveolar rhabdomyosarcoma; cells were described as carrying a p53 mutation, expressing high levels of both myogenin and myoD, and expressing the Pax3/FKHR fusion protein secondary to the t(2;13)(q35;q14) translocation
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
The cell line will only be distributed to non-profit institutions
DSMZ Cell Culture Data:
Medium:
90-95% RPMI 1640 + 5-10% h.i. FBS
Subculture:
seed out at ca. 0.5-1 x 106 cells/80 cm2;
split confluent culture 1:5 to 1:10 every 3-4 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35 hours
Harvest:
cell harvest of ca. 10-15 x 106 cells/80 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Molec. Genetics:
expression of fusion gene PAX3-FOXO1/FKHR confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list