HCC-366
ACC 492
Cell line:
HCC-366
DSMZ no.:
ACC 492
Species:
human (Homo sapiens)
Cell type:
non-small cell lung carcinoma
Origin:
established from the tumor of an 80-year-old woman with non-small cell lung carcinoma (subtype adeno-squamous carcinoma); cells were described in the literature to carry fractional allellic loss with loss of heterozygosity at various hot spots; listed in the CCLE (ref 18232) with extended genome and protein analyses; matched EBV+ B-lymphoblastoid cell line (B-LCL, HCC-366BL) is available (DSMZ ACC 895)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca.0.5-1 x 106 cells/80 cm2; split confluent culture 1:2 to 1:3 once a week using trypsin/EDTA; maximal density at ca. 2 x 106 cells/80 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 72-96 hours
Harvest:
cell harvest of ca. 4 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hyperdiploid karyotype with 12% polyploidy - 48-56<2n>XX, +1, +1, -2, +4, +7, +8, +11, -13, -15, -18, +7-9mar, add(X)(p11), add(1)(p22), add(1)(q11)x1-2, add(4)(q31)x1-2, del(8)(p12), del(8)(q13), add(10)(p13), add(14)(p11), add(17)(p12)
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list