NALM-19
ACC 522
Cell line:
NALM-19
DSMZ no.:
ACC 522
Species:
human (Homo sapiens)
Cell type:
B cell precursor leukemia
Origin:
established from the peripheral blood of a 26-year-old man with "acute undifferentiated leukemia" at diagnosis in 1988
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
cells are difficult to culture; seed out at ca. 3 x 106 cells/ml and culture in 24-well-plates all the time; the first 1-3 weeks after thawing cells do not grow or only very slowly (exchange spent medium with dilution); in growth phase, split saturated culture 1:2 every 2-3 days but do not dilute below 2 x 106 cells/ml; maximal density of ca. 4 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-70 hours
Harvest:
cell harvest of ca. 2-4 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD15 +, CD19 +, CD20 -, CD34 -, CD38 +, cyCD79a +, CD80 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, smkappa -, smlambda -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 4% polyploidy - 47(45-48)<2n>XY,+5, del(9)(p21.2) - revision of published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list