Leibniz Institute DSMZ: Details

Cell line:

JURL-MK2

DSMZ no.:

ACC 533

Species:

human (Homo sapiens)

Cell type:

chronic myeloid leukemia in blast crisis

Origin:

established from the peripheral blood of a 73-year-old man with chronic myeloid leukemia (CML) in blast crisis in 1993; cells carry the Philadelphia chromosome; JURL-MK1 and JURL-MK2 are sister cell lines derived from the same patient

Reference(s):

14459

Biosafety level:

1

Permissions and restrictions:

A

DSMZ Cell Culture Data:

Morphology:

round single cells growing in suspension, heterogenous in size, occasional giant cells; image; image; image

Medium:

85% RPMI 1640 + 15% h.i. FBS

Subculture:

seed out at ca. 0.5 x 10⁶ cells/ml; maintain at ca. 0.2-1.0 x 10⁶ cells/ml; split saturated culture 1:2 to 1:3 every 3-4 days; reduced viability immediately after thawing (ca. 60-70%), but cells will recover thereafter

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 72 hours

Harvest:

cell harvest of ca. 2-3 x 10⁶ cells/ml

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in microbiological culture, PCR assays

Immunology:

CD3 -, CD4 -, CD19 -, CD30 +, CD33 +, HLA-DR +; image

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

human hypotetraploid karyotype with 3.5% polyploidy - 81(77-82)<4n>XXYY, -2, -7, -12, +13, -14, -14, -16, -18, -19, -22, +1-3mar, add(9)(q1)x2, der(9)del(9)(p21.2)t(9;22)(q34;q11)x2, der(11)t(11;9;22)(p15;q34;q11)x2, add(17)(p13)x1-2, der(22)t(9;22)(q34;q11)x2 - carries two copies of Ph effecting BCR-ABL1 fusion together with additional FISH signals on 11p - does not match published karyotype but iteratively resembles JURL-MK1 (sister cell line)

Molec. Genetics:

expression of fusion gene BCR-ABL1 e14-a2 (b3-a2) confirmed by RT-PCR

Viruses:

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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