Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established at diagnosis from the pleural effusion of a 68-year-old man with acute myeloid leukemia (AML M6) secondary to myelodysplastic syndrome (MDS, subtype refractory anemia with excess of blasts, RAEB) in 1989; cells were described in the literature to be proliferatively responsive to GM-CSF and IL-3. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Morphology:
polymorph cells growing as adherent cells and singly in suspension;
image;
image
Medium:
80% RPMI 1640 + 20% h.i. FBS + 10 ng/ml GM-CSF
Subculture:
seed out at ca. 0.3-0.5 x 106 cells/ml; maintain at ca. 1.0 x 106 cells/ml; split saturated culture 1:3 to 1:8 every 2-5 days. Upon cytokine withdrawal cells do not die but undergo a stationary survival status. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines.
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 24-36 hours
Harvest:
cell harvest of ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, CD41 -, CD42 -, CD71 +, CD235a +;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypodiploid karyotype with 6% polyploidy - 41(40-43)<2n>XY, -3, -13, -16, -19, -21, -22, +2mar, ins(X;13)(p11;q?q?), del(3)(p13p24), add(5)(q11), dup(5)(q11q35), der(6)add(6)(p23)dup(6)(q25q27), der(7)add(7)(p13)add(7)(q12), add(9)(q35), der(9)del(9)(q11q34)inv(9)(p2?4;q34), der(10)t(8;10)(q24;p14)t(10;7)(q25;q12), del(11)(q14q21), der(21)t(10;21)(p14;p12) - sideline with dup(1)(p35p36), dup(2)(q1?q2?), del(3)(p13p24) - matches published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
