JEKO-1

human (Homo sapiens)

B cell lymphoma

established from the peripheral blood of a 78-year-old woman with B-cell non-Hodgkin lymphoma (B-NHL), specifically mantle cell lymphoma, large variant in leukemic conversion. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)

14490, 14639, 15850, 18187

1

A

round single cells growing in suspension; image; image

80% RPMI 1640 + 20% h.i. FBS

seed out at ca. 0.5 x 10⁶ cells/ml; maintain at ca. 0.5-1.5 x10⁶ cells/ml; split saturated culture 1:4 twice a week

at 37 °C with 5% CO₂

ca. 50 hours

cell harvest of ca. 1.7 x 10⁶ cells/ml

frozen with 70% medium, 20% FBS, 10% DMSO

contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture, PCR assays

CD3 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylamda -; image

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

human highly rearranged flat-moded hypertriploid karyotype - 70-78<3n>XXXX, +X, +1, +2, +2, +4, +6, +7, -8, +10, +11, -12, -12, +13, +14, +15, -17, +18, -19, -19, -22, +2-4mar, del(X)(p21)/add(X)(p11.2)x2, der(X)t(X;11)(q26-28;q13)t(11;?10)(q24;?p12), der(1)t(1;2)(p21;q22)x2, add(2)(q2?), del(2)(q11), add(3)(q27), der(5)t(5;12)(p15;p11-12)x2, add(6)(p21)x2, der(6;13)(p10;q10)x1-2, der(7)t(4;7)(q2?6;q32.1)x2, add(9)(p2?), der(9)t(9;14)(q34-35;q32-33)x2, der(10)t(10;11)(p12;?)x2, der(11)t(11;14)(q13;q32)x1-2, add(13)(p11), der(13)t(13;?)(p11;?)t(?;13)(?;q11), add(14)(p11), der(14)t(14;17)(p12;q11)t(14;6)(q24;?p11), der(14)t(11;14)(?;p11)t(11;?8)(?;?), del(16)(p11), add(17)(p11), ins(19;12)(q13.1;q13.2q2?4)x2 - carries cryptic rearrangements of CCND1 and IGH (IGH) - related to published karyotype

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Delivery form		Prices
Frozen culture		450,- €
Growing culture   (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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