SU-DHL-5
ACC 571
Cell line:
SU-DHL-5
DSMZ no.:
ACC 571
Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established from a lymph node of a 17-year-old woman with B-cell non-Hodgkin lymphoma (B-NHL), described at the time as diffuse large cell, noncleaved cell type; assigned to GCB-like lymphoma subtype (germinal center B-cell)
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
initially seed out at ca. 0.5 x 106 cells/ml in a 24-well-plate (the viability may drop below 30% after thawing); later, maintain at ca. 0.1-0.8 x 106 cells/ml; split saturated culture 1:2 to 1:5 every 2-3 days; note: viability does usually not exceed 85%
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40 hours
Harvest:
cell harvest of ca. 1.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +
; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 1.5% polyploidy - 47(41-48)<2n>XX, +12, del(6)(q13), del(12)(q13) - sideline with del(6)x2 - matches published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list