REC-1

human (Homo sapiens)

B cell lymphoma

established from the lymph node or peripheral blood from a 61-year-old man with terminal refractory B-cell non-Hodgkin lymphoma (B-NHL, diffuse large cell progressing to transformed mantle cell, blastoid variant). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)

14885, 14892, 15229, 18187

1

A

small round cells growing singly or in clumps in suspension; image; image; image; image

90% RPMI 1640 + 10% h.i. FBS

seed out at ca. 0.6- 1.0 x 10⁶ cells/ml; maintain at ca. 0.4-1.5 x 10⁶ cells/ml; split saturated culture 1:4 to 1:8 every 3-4 days; recommended to start culture after thawing with 20% FBS

at 37 °C with 5% CO₂

ca. 30-40 hours

maximum density at about 3 x 10⁶ cells/ml

frozen with 70% medium, 20% FBS, 10% DMSO

negative in microbiological culture, PCR assays

CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR -, sm/cyIgG -, sm/cy IgM +, sm/cykappa +, sm/cy lambda -; image

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

human highly rearranged flat-moded near-diploid karyotype with 25% polyploidy - 41-47<2n>XY/XXY, -2, -8, +11, +12, -13, -22, add(5)(p15), i(7q), add(9)(p22), der(9)t(9;17)(q3?1;q11-12), add(10)(q24-q25), add(11)(q11), t(11;14)(q13;q32), add(12)(q22), der(12)t(8;12)(q11;p12), der(12)t(12;16)(p11;p11), der(16)t(16;17)(p11;?q25)t(2;16)(p1?2;q2?1), der(16)t(16;22)(p13;q11), add(17)(q25)x2 - carries t(11;14) effecting rearrangement of IGH with CCND1/BCL1 - near tetraploid sideline with additional rearrangements present - matches published description

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Delivery form		Prices
Frozen culture		450,- €
Growing culture   (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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